Hall, J. Christopher, Deschamps, Raymond J. A., & Krieg, Kim K.; “Immunoassays for the detection of 2,4-D and picloram in river water and urine;” Journal of Agricultural and Food Chemistry, 1989, 37(4), 981-984; DOI: 10.1021/jf00088a035.

ABSTRACT:

Immunoassays for 2,4-D [ (2,4-dichlorophenoxy)acetic acid] and picloram (4-amino-3,5,6-trichloro-2pyridinecarboxylic acid) detection were developed with polyclonal antibodies raised in New Zealand white rabbits. Concentrations of 2,4-D within the working range 100-10 000 and 50-10 0oO ng/mL could be quantitated with an indirect enzyme-linked immunosorbent assay (ELISA) and a radioimmunoassay (RIA) in river water and urine, respectively. Concentrations of picloram within the working range 50-5000 ng/mL also could be quantitated in river water and urine by RIA. Determinations using the immunoassays required no sample cleanup. Specificities of the antisera for structurally similar herbicides were low compared to 2,4-D or picloram. The RIA methods incorporated a novel radiolabel consisting of [3H]glycine covalently linked to the herbicide molecule. When compared to the ELISA, the RIA was a more simple, efficient, and rapid procedure, requiring fewer steps to complete the assay. The immunoassays would be suitable for herbicide quantitation in applicator exposure and environmental fate studies. FULL TEXT